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I shouldn't have declared that I only screwed up at work once in a great while - I just did it again.
This time I must have lost cells during the processing of a 96-well plate - When I finished part of a certain procedure, FACS staining, there were some left, but only a mere fraction of what I started out with - enough for what will be done to them next?
F*ck, I hope so, since it's the last experiment so that a paper can be finished, and my boss made a point of asking me to do that procedure as she was having a horrible day, making mistakes, and joked that she shouldn't have gotten out of bed! So she passed it to me...
...and I screwed it up instead. F*cking go me. Maybe partly because I was late for my 2nd job - she'd handed this off to me about 3:30, kind of late...when I realized I'd be late, I let the other job know, but still was under time pressure to not be too teribly late, you know? Worried about catching a bus, etc. I must not have checked the plate after one spin - didn't help that I found out the centrifuge wasn't even working properly; the door was sticking and it may not have spun, with me assuming it did (I swear I always check the plate for pellets, but I must have flaked out and not checked THIS ONE TIME! Or else I saw condensation and mistakenly thought it was a cell pellet - I automatically check, I do! I know it's important not to lose cells. But I somehow must have flicked most of the cells away! NEVER HAVING DONE THAT PARTICULAR MISTAKE BEFORE!
Okay; it isn't the end of the world; maybe there'll be enough. Maybe. I'll know tomorrow.
Another person working with my boss today lost 3 weeks of work when this Idiot tossed the cells she'd just painstakenly collected, thinking they were his BL2 waste.
Three weeks of her work (some of the best cells she'd gotten to date), gone in a careless instant. In my case, a collection of cells of which we have no more (of that specific type at the moment), possibly mostly tossed in the sink by accident, and maybe holding up a paper my boss badly wants published - in a moment.
What did I 'learn' from this? What did she learn from this? That shit happens? In my case, that with two jobs and extra stress due to my current crap time management, this is the price I get for serving too many masters?
Oh, f*cking hurray.
Thanks, Reality.
Oh, and
This time I must have lost cells during the processing of a 96-well plate - When I finished part of a certain procedure, FACS staining, there were some left, but only a mere fraction of what I started out with - enough for what will be done to them next?
F*ck, I hope so, since it's the last experiment so that a paper can be finished, and my boss made a point of asking me to do that procedure as she was having a horrible day, making mistakes, and joked that she shouldn't have gotten out of bed! So she passed it to me...
...and I screwed it up instead. F*cking go me. Maybe partly because I was late for my 2nd job - she'd handed this off to me about 3:30, kind of late...when I realized I'd be late, I let the other job know, but still was under time pressure to not be too teribly late, you know? Worried about catching a bus, etc. I must not have checked the plate after one spin - didn't help that I found out the centrifuge wasn't even working properly; the door was sticking and it may not have spun, with me assuming it did (I swear I always check the plate for pellets, but I must have flaked out and not checked THIS ONE TIME! Or else I saw condensation and mistakenly thought it was a cell pellet - I automatically check, I do! I know it's important not to lose cells. But I somehow must have flicked most of the cells away! NEVER HAVING DONE THAT PARTICULAR MISTAKE BEFORE!
F*CK!
Okay; it isn't the end of the world; maybe there'll be enough. Maybe. I'll know tomorrow.
Another person working with my boss today lost 3 weeks of work when this Idiot tossed the cells she'd just painstakenly collected, thinking they were his BL2 waste.
Three weeks of her work (some of the best cells she'd gotten to date), gone in a careless instant. In my case, a collection of cells of which we have no more (of that specific type at the moment), possibly mostly tossed in the sink by accident, and maybe holding up a paper my boss badly wants published - in a moment.
What did I 'learn' from this? What did she learn from this? That shit happens? In my case, that with two jobs and extra stress due to my current crap time management, this is the price I get for serving too many masters?
Oh, f*cking hurray.
Thanks, Reality.
Oh, and
f*ck you
, Reality..